BioWave  Vol. 21 No. 10
성균관대학교 식품분자생물공학 연구실 우한민 교수
연구실 홈페이지
실험실 소개  
 
성균관대 식품분자생물공학 연구실은 산업미생물의 생리현상 및 대사경로를 이해하고 최신유전공학기술을 이용하여 고부가 화합물의 지속가능한 생산 시스템을 연구하는 대사공학 연구실입니다. 미생물 기반의 화학물질 생산 기술은 기존의 화학공학적 기술보다 좀 더 유연하고 지속가능한 생산이 가능하다는 이점을 갖고 있습니다. 바이오 생리활성물질, 바이오 신소재, 바이오 화합물 등의 다양한 고부가 화합물을 생산하기 위해 혁신적이고 시스테믹한 방법으로 접근하고 있습니다. 또한 합성생물학, 유전자편집기술과 같은 최신 유전자 공학 기술 및 로봇 자동화 시스템을 활용하는 등 최근 연구 동향에 맞추어 끊임없는 진화하는 연구를 진행하고 있습니다.

본 연구실은 체계화된 연구실 운영 시스템을 자랑하고 있으며, 다양한 국내외 학술활동의 기회와 안정된 연구환경을 제공하고 있습니다. 창의력있는 연구원 및 대학원생들이 한 단계 진보할 수 있도록 서로 노력하고 있습니다. 감사합니다.

FMB Lab:smsb at Sungkyunkwan University (SKKU) is a multi-disciplinary laboratory to develop efficient microorganisms to produce valuable chemicals such as biofuels, biomaterials, and biochemicals etc from renewable resources i.e. biomasses. Our lab is running for the number one lab of Metabolic Engineering, Synthetic Biology, Systems Biology.
 

Overview1

Overview2

We are working for scientific research projects to pursue our goals with tools of systems metabolic engineering, microbiology, Omics technologies, molecular biology, synthetic biology and systems biology (est. 2010). We thank you for being interested in our lab.
 
연구분야  
 
1. Metabolically engineered E. coli, C. glutamicum, and cyanobacteria for advanced biofuels and biochemicals
Isoprenoid-based biofuels (Terpenes): Advanced bio-disel and aviation fuel (the next generation biofuels)
Value-added chemicals (>C3 chemicals) production from CO2
Biodiesel and alcohol production from renewable feedstock
Production of biochemicals replacing petroleum-based chemicals.
Production of biochemicals replacing plant extracts. (Cosmetics, pharmaceuticals)

2. Genetic tool development for metabolic engineering using synthetic biology
Systematic gene cloning using BglBrick and Golden Gate method
Systematic gene deletion and integration methods
CRISPR-CAS9 mediated genome editing and CRISPRi-mediated gene controlling
CRISPR-CAS9 mediated Base Editing
Transcriptional and translational controls using RNAs
Genetic re-wiring of control units and biosensors
Its application to industrial host

3. Automatic Metabolic Engineering using Robot Platform
High throughput strain development using Robot Platform
Bioengineering of Robot platform integrated with Genome Editing and Base Editing
Lab. Adaptive Evolution for strain improvement using Robot Platform

4. Systems biology with omics technologies
Genomics - Next generation genome sequencing technology
Transcriptomics - DNA microarray and Next generation seq. technology
Proteomics - MS-based targeted proteomics
Metabolomics - MS-based Metabolite profiling, targeted metablites

5. in silico flux balance analysis
Genome-scale metabolic flux balance analysis
Prediction of metabolic behaviors for metabolic engineering and synthetic biology
Kinetic modeling for cellular phenotypes of co-culture & symbiosis

6. Regulations of cellular metabolisms and physiologies of E. coli and C. glutamicum
Characterization of two-component regulatory systems
in vitro or in situ validation of cellular regulations
Dynamic regulation of gene transcription and translation

7. de Novo pathways to microbe
Biochemical productions from carbon dioxide using engineered Cyanobacteria
Deciphering de Novo pathway in C. glutamicum
 

future biotechnology

 
연구성과  
 

Publications(2018년 이후 논문들)  [#: Co-First Author, *Corresponding Author]

  • Lee, S.S., Park, J.H., Heo, Y.B., *Woo, H.M. (2020)
    Case study of xylose conversion to glycolate in Corynebacterium glutamicum: Current limitation and future perspective of the CRISPR-Cas systems.
    Enzyme Microbial. Technol. , 132:109395

  • Park, J., Riaz, A., Verma, D., Lee, H.J., Woo, H.M., *Kim, J. (2019)
    Fractionation of lignocellulosic biomass over core-shell Ni-alumina catalysts with formic acid as a co-catalyst and hydrogen source.
    ChemSusChem, 12:1743-1762

  • Yook, S.D., Kim, J., Woo, H.M., Um, Y., *Lee, S.M. (2019)
    Efficient lipid extraction from the oleaginous yeast Yarrowia lipolytica using switchable solvents.
    Renewable Energy, 132:61-67

  • Park, J.H., Yu, B.J., Choi. J.-I., *Woo, H.M. (2019)
    Heterologous production of squalene from glucose in engineered Corynebacterium glutamicum using multiplex CRISPR interference and high-throughput fermentation.
    J. Agric. Food Sci., 67(1) 308-319

  • Ko, S.C., Lee, H.J., Choi, S.Y., Choi. J.-I., *Woo, H.M. (2019)
    Bio-solar cell factories for photosynthetic isoprenoids production.
    Planta, 249:181-193

  • Kim, D., *Woo, H.M. (2018)
    Deciphering bacterial xylose metabolism and metabolic engineering of industrial microorganisms for use as efficient microbial cell factories.
    Appl. Microbiol. Biotechnol., 102(22):9471-9480

  • #Ravikumar, S., #Woo, H.M., *Choi, J.I. (2018)
    Analysis of novel antioxidant sesquarterpenes (C35 terpenes) produced in recombinant Corynebacterium glutamicum.
    Appl. Biochem. Biotechnol., 186(3):525-5834

  • Yoon, J. and *Woo, H.M. (2018)
    CRISPR interference-mediated metabolic engineering of Corynebacterium glutamicum for homo-butyrate production.
    Biotechnol. Bioeng., 115(8):2067-2074

  • #Lee, S.S., #Shin, H., Jo, S., Lee, S.M., Um, Y. and *Woo, H.M. (2018)
    Rapid identification of unknown carboxyl esterase activity in Corynebacterium glutamicum using RNA-guided CRISPR interference.
    Enzyme Microb. Technol., 114:63-68

  • Choi, S.Y., Sim, S.J., Choi. J.-I., *Woo, H.M. (2018)
    Identification of small droplets of squalene in engineered Synechococcus elongatus PCC 7942 using TEM and selective fluorescent Nile red analysis.
    Lett. Appl. Microbiol., 66:523-529

  • Kim, E.S., Kim, B.S., Kim, K.Y., Woo, H.M., Lee, S.M., *Um, Y. (2018)
    Aerobic and anaerobic cellulose utilization by Paenibacillus sp. CAA11 and enhancement of its cellulolytic ability by expressing a heterologous endoglucanase.
    J. Biotechnol., 268:21-27

  • Choi, Y.Y., Hong, M.E., Jin E.S., Woo, H.M., *Sim, S.J. (2018)
    Improvement in modular scalability of polymeric thin-film photobioreactor for autotrophic culturing of Haematococcus pluvialis using industrial flue gas.
    Bioresour. Technol., 249:519-526

  • *Woo, H.M. (2018)
    Metabolic pathway rewiring in engineered cyanobacteria for solar-to-chemical and solar-to-fuel production from CO2.
    Bioengineered, 9(1):2-5

  • #Park, J., #Shin, H., Lee, S.M., Um, Y., *Woo, H.M. (2018)
    RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain.
    Microb. Cell Fact., 17:4




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  지도교수: 우한민

 Senior Research Scientist : 이현정
 Post-Docs. : 최선영
 Ph.D. Exchange Students : 나피사
 Ph.D. Students : 이미은, 박재현, 고숭천, 손지경
 Master students : 이유진, 강석원, 임현배, 허유빈, 강두현,
                          이동현, 강동현, 김필, 조혜정, 이성진, 홍예린
Contact : 031-290-7808 (TEL.) / 031-290-7882 (Fax) /
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