DC Biology Assays
evaluate antigenicity and immune modulation with our comprehensive rapid high-throughput DC assay services
Our Dendritic Cell assay platform offers you a comprehensive range of assay types for evaluating the antigenicity and/or immune modulation of novel and biosimilar therapeutic approaches using dendritic cells (DCs).
Our studies typically use monocyte-derived DCs from a range of high resolution HLA-typed human donors from the ProImmune biobank providing you with population-spanning data for your assay results.
ProImmune BioBank Process
The principle of a mixed lymphocyte reaction (MLR) is that T cells from one donor will proliferate in the presence of APCs from a different donor. This is caused by the recognition of an HLA mismatch between two unrelated donors, which provokes an immune response from the T cells. MLR is often used as a means of inducing generalised stimulation/activation of T cells in culture.
Measuring MLR in the presence of an active compound is a useful method for determining the immunomodulatory potential the compound. The advantage of the MLR reaction is that the strong baseline response (reaction without added test compound) does not require stimulation with any other stimulatory compound.
As an example, MLR reactions can be stimulated by co-culture of monocyte-derived dendritic cells with T cells which creates particularly vigorous allogeneic responses.
Following a very similar protocol to our standard ProScern® CFSE DC-T cell proliferation assay, the following data shows example baseline MLR responses in three pairwise mismatched donors (derived from cryopreserved PBMC from healthy donors).
Figure: Flow cytometry plots showing gated live lymphocytes proliferating as a consequence of the MLR reaction. In each case monocyte-derived DCs (top row of figure title) were co-cultured with T cells from a mismatched donor (bottom row of title). In this case proliferation is assessed on CD4+ T cells. Analysing both CD8+ and CD4+ T cell responses is also possible in addition to further phenotyping of cells.
Figure: Assays were carried out in three repeat sets. The values for each set (represented by each dot) represents the average of 8 replicate wells each. MLR responses fell between 60-80% captured CD4+ T cells having undergone proliferation for all donors.
Praise for ProImmune's ProScern® CFSE DC-T Cell Assays by
Dr. Caroline Barelle, Research Director, Shark VNAR Development, University of Aberdeen, UK
"We are developing shark single domain therapeutics so identifying and addressing any immunogenicity issues is a critical part of our pre-clinical compound selection process. ProImmune provided support and information throughout to keep us updated and also succeeded in delivering the data package within our desired and indeed aggressive time frame. Overall we are very happy with the service and would work with ProImmune again".
Mode of action studies
Immune modulation by small molecules