[엘바이오시스템] [ProImmune] Market-leading DC immunobiology assays
분류 : 실험대행기술 > 기타
전화 : 031-229-3350
메일 : lbs_reagents(at).daum.net
팩스 : 031-229-3351
카탈로그 NO
제품 생산일
원산지
생산기업

 

 

DC Biology Assays

evaluate antigenicity and immune modulation with our comprehensive rapid high-throughput DC assay services

 

 

ProScern® DC T cell assays

 

DC MLR assays

 

DC activation assays

 

ProPresent® DC MAPPS assays

 

Request a Quote

 

Dear Colleague,

 

Our Dendritic Cell assay platform offers you a comprehensive range of assay types for evaluating the antigenicity and/or immune modulation of novel and biosimilar therapeutic approaches using dendritic cells (DCs).

 

Our studies typically use monocyte-derived DCs from a range of high resolution HLA-typed human donors from the ProImmune biobank providing you with population-spanning data for your assay results.

 

ProImmune BioBank Process

 

 

 

Request a Quote

 

 

Example: DC MLR Assays

The principle of a mixed lymphocyte reaction (MLR) is that T cells from one donor will proliferate in the presence of APCs from a different donor. This is caused by the recognition of an HLA mismatch between two unrelated donors, which provokes an immune response from the T cells. MLR is often used as a means of inducing generalised stimulation/activation of T cells in culture.  

Measuring MLR in the presence of an active compound is a useful method for determining the immunomodulatory potential the compound. The advantage of the MLR reaction is that the strong baseline response (reaction without added test compound) does not require stimulation with any other stimulatory compound.  

As an example, MLR reactions can be stimulated by co-culture of monocyte-derived dendritic cells with T cells which creates particularly vigorous allogeneic responses.

Following a very similar protocol to our standard ProScern® CFSE DC-T cell proliferation assay, the following data shows example baseline MLR responses in three pairwise mismatched donors (derived from cryopreserved PBMC from healthy donors).

 

Representative Flow Cytometry Plots

 

Figure: Flow cytometry plots showing gated live lymphocytes proliferating as a consequence of the MLR reaction. In each case monocyte-derived DCs (top row of figure title) were co-cultured with T cells from a mismatched donor (bottom row of title). In this case proliferation is assessed on CD4+ T cells. Analysing both CD8+ and CD4+ T cell responses is also possible in addition to further phenotyping of cells.

 

Figure: Assays were carried out in three repeat sets. The values for each set (represented by each dot) represents the average of 8 replicate wells each. MLR responses fell between 60-80% captured CD4+ T cells having undergone proliferation for all donors.

 

Praise for ProImmune's ProScern® CFSE DC-T Cell Assays by 

Dr. Caroline Barelle, Research Director, Shark VNAR Development, University of Aberdeen, UK

"We are developing shark single domain therapeutics so identifying and addressing any immunogenicity issues is a critical part of our pre-clinical compound selection process. ProImmune  provided support and information throughout to keep us updated and also succeeded in delivering the data package within our desired and indeed aggressive time frame. Overall we are very happy with the service and would work with ProImmune again".

 

 

Request a Quote

 

 

More on

ProScern® DC T cell assays

 

DC MLR assays

 

DC activation assays

 

ProPresent® DC MAPPS assays

 

ProMap® petide T cell assays

Recent Projects

Immuno oncology

Autoimmune diseases

Infectious diseases

Ophthalmology

Hematology

Respiratory diseases

Consumer products

 

Application Areas

Protein design

Lead selection

Mode of action studies

Formulation

Batch release

Manufacturing development

Comparing biosimilars

Immune modulation by small molecules

 

 

https://www.lbiosystems.co.kr/   

 

 

 

 

주소복사
HOME   |   이용약관   |   개인정보처리방침
© BRIC