BioWave  Vol. 20 No. 9
성균관대학교 발생생식분자생물학 연구실 오정수 교수
연구실 홈페이지
실험실 소개  
발생생식분자생물학 연구실은 생식세포 및 초기 배아 발생과정에서 일어나는 일련의 생명현상을 분자적인 수준에서 연구하는 실험실입니다. 특히나 난자는 발생과 동시에 감수분열 초기단계에서 세포주기가 멈춰있게 됩니다. 따라서, 세포주기 조절기작을 연구하기 위한 중요한 모델시스템으로써 활용될 수 있습니다.

본 연구실은 난자를 통해서 새로운 세포주기 조절인자를 탐색하고 이를 암세포와 같은 체세포에 응용함으로써 새로운 항암치료제를 개발하고자 합니다. 또한, 초기 배아 발생과정에서 일어 나는 유전자의 리프로그래밍 기작을 연구함으로써 줄기세포의 유전자 조절기작을 이해하고자 합니다.

lab_intro

연구내용  
Our research focuses on the molecular mechanisms that regulate gamete maturation, fertilization, and preimplantation development. We are also interested in the epigenetic reprogramming during the egg-to-embryo transition.

1. Regulation of meiotic cell cycle
Cell division is a highly regulated process, driven by the action of cyclin-dependent kinases (Cdks) and their regulatory subunits, cyclins. Inappropriate activation of Cdk/cyclin complexes can drive unregulated cell division resulting in cell death or tumor formation. Cdk/cyclin complexes also play essential roles in a variety of cellular processes such as development and differentiation. Our lab is focused on the action of Cdk/cyclin complexes and all pathways that control or are controlled by Cdk/cyclin complexes during female meiosis.

2. Homologous chromosome segregation and aneuploidy
Reduction of chromosome number during meiosis is essential for producing haploid gametes from diploid parental cells. This reduction is achieved by two successive rounds of chromosome segregation, meiosis I (MI) and meiosis II (MII), after a single round of DNA replication. Although MII resembles mitosis in that sister chromatids separate and segregate to different daughter cells, the pattern of chromosome segregation during MI is unique. During MI, homologous chromosomes pair and then segregate from each other. Defects in this process result in aneuploidy, leading to miscarriages, infertility and genetic disorders such as Down’s syndrome. Therefore, our lab studies the molecular mechanisms that control homologous chromosome segregation during meiosis.

3. Control of oocyte quality
Mammalian oocytes are arrested at the prophase of the first meiosis. After LH surge, the oocytes resume meiosis. During these processes, the oocytes is subjected to various sources of damage-inducing factors, which may lead to a progressive deterioration of oocyte quality. Thus, the control of oocyte quality is critical to reproductive success and survival of a species; however, the precise mechanisms underlying this process remain elusive. Our lab is interesting in identifying the molecular mechanisms that control oocyte quality and eventually leading to identification of diagnostic markers that are predictive of oocyte quality in a clinical setting.

4. Chromatin remodeling during fertilization
Fertilization leads to the transformation of two haploid gametes into a totipotent zygote. During this process, highly condensed chromatins are largely reorganized, preparing to initiate the transcription of embryonic genes which are essential for obtaining the pluripotency. Our lab is exploring the mechanisms of the chromatin remodeling upon fertilization and investigating the role of maternal factors in this process.

연구성과  
2016년 이후 연구논문들

  • Yoon YN, Choe MH, Jung KY, Hwang SG, Oh JS*, Kim JS* (2018)
    MASTL inhibition promotes mitotic catastrophe through PP2A activation to inhibit cancer growth and radioresistance in breast cancer cells.
    BMC Cancer, 18(1):716 (*corresponding)

  • Choe MH, Kim J, Ahn J, Hwang SG, Oh JS, Kim JS. (2018)
    Centrosome Clustering Is a Tumor-selective Target for the Improvement of Radiotherapy in Breast Cancer Cells.
    Anticancer Res., 38(6):3393-3400

  • Leem J, Kim JS, Oh JS* (2018)
    WIP1 phosphatase suppresses the DNA damage response during G2/prophase arrest in mouse oocytes.
    Biol Reprod., (*corresponding)

  • Wang H, Choe MH, Lee IW, Namgoong S, Kim JS*, Kim NH*, Oh JS* (2017)
    CIP2A acts as a scaffold for CEP192-mediated microtubule organizing center assembly by recruiting Plk1 and aurora A during meiotic maturation.
    Development, 144(20):3829-3839, (*corresponding)

  • Kim MO, Choe MH, Yoon YN, Ahn J, Yoo M, Jung KY, An S, Hwang SG, Oh JS*, Kim JS* (2017)
    Antihelminthic drug niclosamide inhibits CIP2A and reactivates tumor suppressor protein phosphatase 2A in non-small cell lung cancer cells.
    Biochem Pharmacol., 144:78-89, (*corresponding)

  • Jeon HJ, You SY, Kim DH, Jeon HB, Oh JS* (2017)
    Protective effects of ethanol extracts of Artemisia asiatica Nakai ex Pamp. on ageing-induced deterioration in mouse oocyte quality.
    Zygote, 25(4):472-479, (*corresponding)

  • Jeon HJ, Park YS, Cho DH, Kim JS, Kim E, Chae HZ, Chun SY*, Oh JS* (2017)
    Peroxiredoxins are required for spindle assembly, chromosome organization, and polarization in mouse oocytes.
    Biochem Biophys Res Commun., 489(2):193-199, (*corresponding)

  • Wang H, Guo J, Lin Z, Namgoong S, Oh JS*, Kim NH* (2017)
    Filamin A is required for spindle migration and asymmetric division in mouse oocytes.
    FASEB J., 31(8):3677-3688, (*corresponding)

  • Jeon HJ, Cui XS, Guo J, Lee JM, Kim JS, Oh JS* (2017)
    TCTP regulates spindle assembly during postovulatory aging and prevents deterioration in mouse oocyte quality.
    Biochim Biophys Acta., 1864(7):1328-1334, (*corresponding)

  • Wang H, Jo YJ, Oh JS*, Kim NH* (2017)
    Quercetin delays postovulatory aging of mouse oocytes by regulating SIRT expression and MPF activity.
    Oncotarget, 8:38631-38641, (*corresponding)

  • Cho S, Roh K, Park J, Park YS, Lee M, Cho S, Kil EJ, Cho MJ, Oh JS, Byun HS, Cho SH, Park K, Kang H, Koo J, Yeom CH, Lee S (2017)
    Hydrolysis of Hyaluronic Acid in Lymphedematous Tissue Alleviates Fibrogenesis via TH1 Cell-Mediated Cytokine Expression.
    Sci Rep., 7(1):35

  • Wang H, Jo YJ, Sun TY, Namgoong S, Cui XS, Oh JS*, Kim NH* (2016)
    Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosis.
    Biochim Biophys Acta., 1863(12):2993-3000, (*corresponding)

  • Jo DS, Shin DW, Park SJ, Bae JE, Kim JB, Park NY, Kim JS, Oh JS, Shin JW, Cho DH (2016)
    Attenuation of Aβ toxicity by promotion of mitochondrial fusion in neuroblastoma cells by liquiritigenin.
    Arch Pharm Res., 39(8):1137-43

  • Park YS, You SY, Cho S, Jeon HJ, Lee S, Cho DH, Kim JS, Oh JS* (2016)
    Eccentric localization of catalase to protect chromosomes from oxidative damages during meiotic maturation in mouse oocytes.
    Histochem Cell Biol., 146(3):281-8, (*corresponding)

  • You SY, Park YS, Jeon HJ, Cho DH, Jeon HB, Kim SH, Chang JW, Kim JS*, Oh JS* (2016)
    Beclin-1 knockdown shows abscission failure but not autophagy defect during oocyte meiotic maturation.
    Cell Cycle, 15(12):1611-9, (*corresponding)

  • Jeon HJ, You SY, Park YS, Chang JW, Kim JS, Oh JS* (2016)
    TCTP regulates spindle microtubule dynamics by stabilizing polar microtubules during mouse oocyte meiosis.
    Biochim Biophys Acta., 1863(4):630-7, (*corresponding)

  • Kim JS, Kim HA, Seong MK, Seol H, Oh JS, Kim EK, Chang JW, Hwang SG, Noh WC (2016)
    STAT3-survivin signaling mediates a poor response to radiotherapy in HER2-positive breast cancers.
    Oncotarget, 7(6):7055-65

  • Park HJ, Oh JS, Chang JW, Hwang SG, Kim JS (2016)
    Proton Irradiation Sensitizes Radioresistant Non-small Cell Lung Cancer Cells by Modulating Epidermal Growth Factor Receptor-mediated DNA Repair.
    Anticancer Res., 36(1):205-12

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     박사후연구원 : GUANGYU BAI
     박사과정 : 송시진, 전혁준
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     석사과정 : 박유람

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