BioWave,  Vol. 20 No. 8
국민대학교 시스템생물학 연구실 이도엽 교수
연구실 홈페이지
실험실 소개  
Often, a multitude of biochemical responses are triggered in order to overcome or counteract genetic/environmental perturbation. In addition, the intensity and the time lag for such responses reflect the biochemical network that is laid out by the genetic and structural constraints of a given biological system. We want to study which metabolic rescue mechanisms are triggered in response to various genetic and environmental stimuli (e.g. disease, nutritional stress) and how the metabolic modulation is related.

Here, we apply various types of mass-spectrometry platforms and bioinformatics approaches to unveil & link multi-layered molecular constituents, metabolite and protein to the relevant metabolic physiology. We hope the investigation can facilitate comprehensive understanding of disease mechanism, discover novel biomarkers, therapeutic compounds, and provide roadmap to re-construct newly designed microorganism for valuable compounds.


1. Clinical Metabolomis
One of our researches is focused on determining the metabolic causality induced by various types of human disease. By examining the modulation of broad range of small molecules, “metabolites” (Metabolomics) and proteins (Proteomics), we aim at understanding the biochemical mechanistics and metabolic phenotypes of such diseases as neurodegenerative disease, kidney disease, diabetics, and obesity. Further, we hope to develop diagnostic and prognostic biomarker, pharmacological and molecular approaches to treating human disease.

Clinical Metabolomis

2. Microbial Metabolomics
Microbe is very first target of many scientific researches as model organism of more complex biological system (e.g. animals and plants). In addition, microorganism has been attractive resources which can produce biologically-functional compounds (e.g. prototypes of drugs, bioactive compounds), and industrially-valuable materials. In our lab, we utilize Chlamydomonas, a unicellular alga that is very well suited for such complex studies: it is a robust organism that occurs naturally in various environments, its genome is sequenced, and genetic tools and mutant and RNAi strains are available through the Chlamydomonas research community. It is biochemically related to other Eukaryotes like yeast but it also shares biochemical relationships to higher plants, like a chloroplast and starch synthesis. We aim at exploiting a variety of cutting-edge technologies in mass spectrometry and statistics to identify and quantify large metabolic networks for both metabolites and soluble enzymes.

Microbial Metabolomics

3. Food Science
We are also interested at metabolomics-based food research in which we explore novel bioactive compounds from natural product, assess food safety, and quality using high-throughput mass-spectrometry analysis.

Methionine restriction and aging

4. Others
We are open to your own nice idea whichever has "metabolism" in it. Bring your idea to "Systems"

2015년 이후 연구논문들

  • Dong Yoon Ji, Se Hee Park, Soo Jin Park, Kyoung Heon Kim, Cheol Ryong Ku, Dong Yeob Shin, Jin Sook Yoon, Do Yup Lee & Eun Jig Lee (2018)
    Comparative assessment of Graves’ disease and main extrathyroidal manifestation, Graves’ ophthalmopathy, by non-targeted metabolite profiling of blood and orbital tissue.
    Sci. Rep., 8:9262

  • Yeong-Je Seong, Hye-Jin Lee, Jung-Eun Lee, Sooah Kim, Do Yup LEE, Kyoung Heon Kim, Yong-Cheol Park (2017)
    Physiological and metabolomic analysis of Issatchenkia orientalis MTY1 with multiple tolerance for cellulosic bioethanol production.
    Biotechnol. J., 12(11):1700110

  • Yeo Ul Cho, Deokjong Lee, Jung-Eun Lee, Kyoung Heon Kim, Do Yup Lee, Young-Chul Jung (2017)
    Exploratory metabolomics of biomarker identification for the internet gaming disorder in young Korean males.
    J. Chromatogr. B, 1057:24-31

  • Cheol-Ho Jang, Gayeon Lee, Yong-Cheol Park, Kyoung Heon Kim, Do Yup Lee (2017)
    Highly Time-Resolved Metabolic Reprogramming toward Differential Levels of Phosphate in Chlamydomonas reinhardtii.
    J. Microbiol. Biotechnol., 27(6):1150-1156

  • Soo Jin Park, In Hye Jeong, Byung Soo Kong, Jung-Eun Lee, Kyoung Heon Kim, Do Yup Lee, Ho Jin Kim (2016)
    Disease type-and status-specific alteration of CSF metabolome coordinated with clinical parameters in inflammatory demyelinating diseases of CNS.
    PloS one, 11(11):e0166277

  • Jung-Eun Lee, Yeo Ul Cho, Kyoung Heon Kim, Do Yup Lee (2016)
    Distinctive metabolomic responses of Chlamydomonas reinhardtii to the chemical elicitation by methyl jasmonate and salicylic acid.
    Process Biochem., 51(9):1147-1154

  • Jung-Eun Lee, Yu Ho Lee, Se-Yun Kim, Yang Gyun Kim, Ju-Young Moon, Kyung-Hwan Jeong, Tae Won Lee, Chun-Gyoo Ihm, Sooah Kim, Kyoung Heon Kim, Dong Ki Kim, Yon Su Kim, Chan-Duck Kim, Cheol Whee Park, Do Yup Lee, Sang-Ho Lee (2016)
    Systematic biomarker discovery and coordinative validation for different primary nephrotic syndromes using gas chromatography-mass spectrometry.
    J. Chromatogr. A, 1453:105-115

  • Jung-Eun Lee, Yunjung Kim, Kyoung Heon Kim, Do Yup Lee, Youngseok Lee (2016)
    Contribution of drosophila TRPA1 to metabolism.
    PloS one, 11(4):e0152935

  • Son HH, Lee DY, Seo HS, Jeong J, Moon JY, Lee JE, Chung BC, Kim E, Choi MH. (2016)
    Hair sterol signatures coupled to multivariate data analysis reveal an increased 7β-hydroxycholesterol production in cognitive impairment.
    J. Steroid Biochem. Mol. Biol., 155(A):9-17

  • YH Cho, HJ Lee, JE Lee, SJ Kim, K Park, DY Lee, YC Park (2015)
    Fast determination of multiple-reaction intermediates for long-chain dicarboxylic acid biotransformation by gas chromatography-flame ionization detector.
    J. Microbiol. Biotechnol., 25(5):704-708

      지도교수: 이도엽

     PhD student : 이정은, 박수진
     Master student : 이은미, 이창완, 이보미, 강유진, 권형도, 유정석

    Contact : 02-910-5644 (Tel.) /
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    등록일 2018.07.27
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