BioWave  Vol. 20 No. 7
고려대학교 바이오분자공학실험실 이병천 교수
연구실 홈페이지
실험실 소개  
Cellular components in aerobic organisms are often confronted with reactive oxygen species (ROS). ROS may damage various biomolecules, such as DNA, proteins, and lipids, and may contribute to the development of cancer, neurodegenerative diseases and other maladies. It also accelerates aging. Sulfur-containing amino acids including methionine are the major targets of ROS and thus the amino acids most susceptible to oxidation. Up-to-date, many enzymes such as Mical and Msr are known to be involved in oxidation and reduction of methionine and methionine sulfoxide, respectively.

Our lab aims to solve mysteries in the regulation of biological functions by utilizing reversible methionine oxidation/reduction by Mical and Msr. We are seeking to understand its underlying mechanism, relevant enzymes, physiological role, and disease association caused by malfunctions. In addition, we are seeking to find a biotechnological application of Msr as a biosensor. This system will be used to monitor real-time oxidative stress change inside cells and detect specific disease conditions. Finally, our lab strives to find the secret of aging by using two model systems, yeast and fruit fly. In particular, we have focused on lifespan extension by methionine restriction and regulation of sulfur metabolism.
연구내용  
1. Structure & function of Msr/Mical proteins
Msr(Methionine sulfoxide reductase) 단백질의 항산화 기전과 단백질 기능 조절 기전에 관한 연구
Mical 단백질의 구조 및 methionine 산화 기전 연구
MsrB1에 의한 초기면역 조절 기전 및 관련질병모델 연구

Structure & function of Msr/Mical proteins

2. Biosensor for methionine oxidation
타겟 단백질내 methionine 산화를 정량적으로 검출할 수 있는 fluorescence-based biosensor 개발
세포내 산화스트레스의 변화를 실시간으로 모니터링 할 수 있는 real-time biosensor 개발
Biosensor를 탑재한 모델동물 개발 및 노화기전연구에 응용

Biosensor for methionine oxidation

3. Methionine restriction and aging
Methionine restriction diet에 의한 수명연장기전 연구
Sulfur metabolism에 의한 활성산소의 발생 조절 및 노화에 대한 영향 연구
노화방지 및 수명연장을 유도하는 식품 및 신약의 개발

Methionine restriction and aging

연구성과  
2014년 이후 연구논문들

  • Ma S, Avanesov AS, Porter E, Lee BC, Mariotti M, Zemskaya N, Guigo R, Moskalev AA, Gladyshev VN. (2018)
    Comparative transcriptomics across 14 Drosophila species reveals signatures of longevity.
    Aging Cell, e12740

  • Lee BC*, Lee HM, Kim S, Avanesov AS, Lee A, Chun BH, Vorbruggen G, Gladyshev VN. (2018)
    Expression of the methionine sulfoxide reductase lost during evolution extends Drosophila lifespan in a methionine-dependent manner.
    Sci Rep., 8:1010 (*: Co-corresponding author)

  • Lee BC*, Lee S, Choo M, Kim JH, Lee HM, Kim S, Fomenko DE, Kim H, Park JM, and Gladyshev VN* (2017)
    Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo.
    Sci Rep., 7:5119 (*: Co-corresponding author)

  • Lee SY, Lee HY, Song JH, Kim GT, Jeon S, Song YJ, Lee JS, Hur JH, Hee Oh H, Park SY, Shim SM, Yoo HJ, Lee BC, Jiang XC, Choi CS, Park TS. (2017)
    Adipocyte-Specific Deficiency of de novo Sphingolipid Biosynthesis Leads to Lipodystrophy and Insulin Resistance.
    Diabetes, 66(10):2596-2609

  • Truong HA, Jeong CY, Lee WJ, Lee BC, Chung N, Kang CS, Cheong YK, Hong SW, Lee H. (2017)
    Evaluation of a Rapid Method for Screening Heat Stress Tolerance Using Three Korean Wheat (Triticum aestivum L.) Cultivars.
    J Agric Food Chem., 65:5589-5597

  • Gladyshev VN, Arner ES, Berry MJ, Brigelius-Flohe R, Bruford EA, Burk RF, Carlson BA, Castellano S, C`havatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Flohe L, Green FR, Guigo R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, Kohrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Prabhu KS, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovic M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y. (2016)
    Selenoprotein Gene Nomenclature.
    J Biol Chem., 291:24036-24040

  • Cox AG, Tsomides A, Kim AJ, Saunders D, Hwang KL, Evason KJ, Heidel JR, Brown KK, Yuan M, Lien EC, Lee BC, Nissim S, Dickinson BC, Chhangawala S, Chang CJ, Asara JM, Houvras Y, Gladyshev VN, Goessling W (2016)
    Selenoprotein H is an essential regulator of redox homeostasis that cooperates with p53 in development and tumorigenesis.
    PNAS, 113:E5562-71

  • Lee BC*, Kaya A, and Gladyshev VN* (2016)
    Methionine restriction and lifespan control.
    Ann N Y Acad Sci., 1363:116-124 (*: Co-corresponding author)

  • Kaya A, Lee BC*, and Gladyshev VN* (2015)
    Regulation of protein function by reversible methionine oxidation and the role of selenoprotein MsrB1.
    Antioxid. Redox Signal, 23: 814-822 (*: Co-corresponding author)

  • Kim MJ, Lee BC, Hwang KY, Gladyshev VN and Kim HY (2015)
    Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine.
    Biochem. Biophys. Res. Commun., 461:648-652

  • Tarrago L*, Peterfi Z*, Lee BC, Michel T and Gladyshev VN (2015)
    Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors.
    Nat. Chem. Biol., 11:332-338 (*: Equal contribution)

  • Hine C, Harputlugil E, Zhang Y, Ruckenstuhl C, Lee BC, Brace L, Longchamp A, Trevino-Villarreal JH, Mejia P, Ozaki CK, Wang R, Gladyshev VN, Madeo F, Mair WB, and Mitchell JR (2015)
    Endogenous hydrogen sulfide production is essential for dietary restriction benefits.
    Cell, 160:132-144

  • Avanesov A, Ma, S, Pierce K, Yim SH, Lee BC, Clish CB and Gladyshev VN (2014)
    Age- and diet-associated metabolome remodeling characterizes the aging process driven by damage accumulation.
    Elife, 3:e02077

  • Han S*, Lee BC*, Yim SH, Gladyshev VN and Lee S (2014)
    Characterization of mammalian selenoprotein O: A redox active selenoprotein in mitochondria.
    PLoS One, 9:e95518 (*: Equal contribution)

  • Lee BC, Kaya A, Ma S, Kim G, Gerashchenko MV, Yim SH, Hu Z, Harshman LG and Gladyshev VN (2014)
    Methionine restriction extends lifespan of drosophila melanogaster under conditions of low amino-acid status.
    Nat. Commun., 5:3592

  •  
    멤버사진
    확대보기  
      지도교수: 이병천

     박사과정 : 이해민
     석사과정 : 이아로, 김민서, 김세현
     학부연구생 : 노연진

    Contact : 02-3290-3529 (Lab) / Email
    Homepage
     
    < 본 정보는 해당 대학/연구실에서 제공하는 자료를 바탕으로 구성된 내용으로, BRIC에서 작성한 정보가 아님을 밝힙니다. >
    등록 2018.07.02
    주소복사
    © BRIC