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The lateral flow immunosensor (LFI) is a widely used diagnostic tool for biomarker detection; however, its sensitivity is often insufficient for analyzing targets at low concentrations. Here, an electrochemiluminescent LFI (ECL‐LFI) is developed for highly sensitive detection of troponin I (TnI) using Ru(bpy)32+‐loaded mesoporous silica nanoparticles (RMSNs). A large amount of Ru(bpy)32+ is successfully loaded into the mesoporous silica nanoparticles with excellent loading capacity and shows strong ECL signals in reaction to tripropylamine. Antibody‐immobilized RMSNs are applied to detect TnI by fluorescence and ECL analysis after a sandwich immunoassay on the ECL‐LFI strip. The ECL‐LFI enables the highly sensitive detection of TnI‐spiked human serum within 20 min at femtomolar levels (≈0.81 pg mL−1) and with a wide dynamic range (0.001–100 ng mL−1), significantly outperforming conventional fluorescence detection (>3 orders of magnitude). Furthermore, TnI concentrations in 35 clinical serum samples across a low range (0.01–48.31 ng mL−1) are successfully quantified with an excellent linear correlation (R2 = 0.9915) using a clinical immunoassay analyzer. These results demonstrate the efficacy of this system as a high‐performance sensing strategy capable of capitalizing on future point‐of‐care testing markets for biomolecule detection.
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학술웨비나 - 연구성과
High-amplitude cofluctuation의 대뇌 functional connectivity에서의 역할 [Proc. Natl. Acad. Sci. USA]
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개최일시 : 1월 18일 (월) 오전 10시 (한국시간)
발표연사 : 조영흔 (Indiana University)
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학술웨비나 - 연구성과
H3K27M을 지닌 소아뇌종양에서 신진대사와 후성유전학의 관계 [Cancer Cell]
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개최일시 : 2월 4일 (목) 오전 11시 (한국시간)
발표연사 : 정찬 (University of Michigan)
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학술웨비나 - 연구성과
약물유전단백체학을 활용한 교모세포종 (Glioblastoma) 연구 [Nat. Commun.]
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개최일시 : 2월 16일 (화) 오후 02시 (한국시간)
발표연사 : 오세진 (연세대학교 의과대학)
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학술웨비나 - 연구성과
스무색 만으로 완성하지 못하는 단백질 디자인 [Nat. Commun.]
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개최일시 : 2월 17일 (수) 오전 10시 (한국시간)
발표연사 : 이준구 (Northwestern University)
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