전형민 (Hyungmin Jun) Massachusetts Institute of Technology
CV (166.65 KB)
이메일
Nat. Commun., Published 28 November 2019, 10, Article number: 5419 | DOI https://doi.org/10.1038/s41467-019-13457-y
Automated sequence design of 2D wireframe DNA origami with honeycomb edges
펼치기 Authors and Affiliations
Abstract

Wireframe DNA origami has emerged as a powerful approach to fabricating nearly arbitrary 2D and 3D geometries at the nanometer-scale. Complex scaffold and staple routing needed to design wireframe DNA origami objects, however, render fully automated, geometry-based sequence design approaches essential for their synthesis. And wireframe DNA origami structural fidelity can be limited by wireframe edges that are composed only of one or two duplexes. Here we introduce a fully automated computational approach that programs 2D wireframe origami assemblies using honeycomb edges composed of six parallel duplexes. These wireframe assemblies show enhanced structural fidelity from electron microscopy-based measurement of programmed angles compared with identical geometries programmed using dual-duplex edges. Molecular dynamics provides additional theoretical support for the enhanced structural fidelity observed. Application of our top-down sequence design procedure to a variety of complex objects demonstrates its broad utility for programmable 2D nanoscale materials.
 

Category: Biophysics, Nanobio
등록일 2019.12.02
주소복사
댓글 (0)
© BRIC