석영재 (Yeong-Jae Seok) 서울대학교 생명과학부
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Nat. Commun., Published : 25 November 2019, 10, Article number: 5358 | DOI https://doi.org/10.1038/s41467-019-13353-5
Sugar-mediated regulation of a c-di-GMP phosphodiesterase in Vibrio cholerae
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Abstract

Biofilm formation protects bacteria from stresses including antibiotics and host immune responses. Carbon sources can modulate biofilm formation and host colonization in Vibrio cholerae, but the underlying mechanisms remain unclear. Here, we show that EIIAGlc, a component of the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS), regulates the intracellular concentration of the cyclic dinucleotide c-di-GMP, and thus biofilm formation. The availability of preferred sugars such as glucose affects EIIAGlc phosphorylation state, which in turn modulates the interaction of EIIAGlc with a c-di-GMP phosphodiesterase (hereafter referred to as PdeS). In a Drosophila model of V. cholerae infection, sugars in the host diet regulate gut colonization in a manner dependent on the PdeS-EIIAGlc interaction. Our results shed light into the mechanisms by which some nutrients regulate biofilm formation and host colonization.

Category: Biochemistry, Physiology, Microbiology
등록일 2019.11.28
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