University of Cambridge

CV(PDF)

이메일
Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells
펼치기 Authors and Affiliations
Abstract

The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of “punctuated” neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.

Keywords : gastric corpus isthmus stem cell; two stem cell compartments; punctuated neutral drift; unbiased genetic labeling; deep tissue imaging; biophysical modeling; single-cell RNA-seq; Lgr5; Troy; intestine

주소복사
논문정보   
- 형식: Research article
- 게재일: 2019년 08월 (BRIC 등록일 2019-08-20)
- 연구진: 국내(교신)+국외 연구진태극기
- 분야: Cell_Biology
댓글 (0)
웨비나 참석자 모집중...
HOME   |   이용약관   |   개인정보처리방침
© BRIC