Northwestern University, 현 Rutgers, The State University of New Jersey


Nanopatterning the Chemospecific Immobilization of Cowpea Mosaic Virus Capsid
Jennifer C. Smith,†,| Ki-Bum Lee,‡,| Qian Wang,§,| M. G. Finn,§ John E. Johnson,§ Milan Mrksich,*,† and Chad A. Mirkin*,‡

Department of Chemistry and The Institute for Biophysical Dynamics, The UniVersity of Chicago, 5735 South Ellis AVenue, Chicago, Illinois 60637, Department of Chemistry and The Institute for Nanotechnology, Northwestern UniVersity, 2145 Sheridan Road, EVanston, Illinois 60208-3113, and Departments of Chemistry and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037

Received December 19, 2002; Revised Manuscript Received March 20, 2003

This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary inkconsisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one maleimide groupto pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.
- 형식: Research article
- 게재일: 2003년 06월 (BRIC 등록일 2014-12-24)
- 연구진: 국외연구진
- 피인용횟수: 120회 이상 인용된 논문
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