Nanopatterning the Chemospecific Immobilization of Cowpea Mosaic Virus Capsid
Jennifer C. Smith,†,| Ki-Bum Lee,‡,| Qian Wang,§,| M. G. Finn,§ John E. Johnson,§ Milan Mrksich,*,† and Chad A. Mirkin*,‡
Department of Chemistry and The Institute for Biophysical Dynamics, The UniVersity of Chicago, 5735 South Ellis AVenue, Chicago, Illinois 60637, Department of Chemistry and The Institute for Nanotechnology, Northwestern UniVersity, 2145 Sheridan Road, EVanston, Illinois 60208-3113, and Departments of Chemistry and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037
Received December 19, 2002; Revised Manuscript Received March 20, 2003
This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary inkconsisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one maleimide groupto pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.