Northwestern University, 현 Rutgers, The State University of New Jersey

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The Use of Nanoarrays for Highly Sensitive and Selective Detection of Human Immunodeficiency Virus Type 1 in Plasma

Ki-Bum Lee , Eun-Young Kim , Chad A. Mirkin ,*† and Steven M. Wolinsky *†‡

Department of Chemistry and The Institute for Nanotechnology, Northwestern University, Evanston, Illinois, and Division of Infectious Diseases, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois

* Corresponding authors.Chad A. Mirkin, Steven M. Wolinsky

Department of Chemistry and The Institute for Nanotechnology.
The Feinberg School of Medicine.

Abstract
Arrays of antibodies with well-defined feature size and spacing are necessary for developing highly sensitive and selective immunoassays to detect macromolecules in complex solutions. Here we report the application of nanometer-scale antibody array-based analysis to determine the presence of the human immunodeficiency virus type 1 (HIV-1) in blood samples. Dip-pen nanolithography (DPN) was used to generate nanoscale patterns of antibodies against the HIV-1 p24 antigen on a gold surface. Feature sizes were less than 100-nanometers, and the activity of the antibody was preserved. HIV-1 p24 antigen in plasma obtained directly from HIV-1-infected patients was hybridized to the antibody array in situ, and the bound protein was hybridized to a gold antibody-functionalized nanoparticle probe for signal enhancement. The nanoarray features in the three-component sandwich assay were confirmed by atomic force microscopy (AFM). Demonstration of measurable amounts of HIV-1 p24 antigen in plasma obtained from men with less than 50 copies of RNA per ml of plasma (corresponding to 0.025 pg per ml) illustrates that the nanoarray-based assay can exceed the limit of detection of conventional enzyme-linked immunosorbent assay (ELISA)-based immunoassays (5 pg per ml of plasma) by more than 1000-fold.

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논문정보   
- 형식: Research article
- 게재일: 2004년 10월 (BRIC 등록일 2013-05-09)
- 연구진: 국외연구진
- 분야: Biochemistry
- 피인용횟수: 120회 이상 인용된 논문
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