Protein Nanoarrays Generated By Dip-Pen Nanolithography
Ki-Bum Lee,(1)1So-Jung Park,(1)Chad A. Mirkin,(1)*Jennifer C. Smith,(2) Milan Mrksich(2)*
Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy. As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.
(1) Northwestern University, Department of Chemistry and Center for Nanofabrication and Molecular Self-Assembly, 2145 Sheridan Road, Evanston, IL 60208, USA.
(2)University of Chicago, Department of Chemistry and the Institute for Biophysical Dynamics, 5735 South Ellis, Chicago, IL 60637, USA.
* To whom correspondence should be addressed.