2+1 행사 !! ECL!! / nanostring x illumina seminar / NEB High Molecular Weight DNA Extraction kit / SplintR ligase를 이용한 miRNA 검출 / 실험 준비가 간편한 저산소 세포배양 키트 / Novogene NGS Service
등록일   2020.12.09  |  필코리아테크놀로지
마감일 ~ 2020.12.08 까지

 

                  

 

0.2+1!! 잘 안 잡히는 단백질, advansta의 강력한 WesternBright™ ECL로 해결하세요.

 

 

1. nanoString&Illumina Joint Seminar나노스트링 GeoMx Digital Spatial Profiler 세미나에 참석하세요

Join Us at The Year End Asia Pacific Spatial Genomics Summit
2020 is approaching its end and it is time to celebrate the innovation and breakthrough in Spatial Biology!
 
Spatial technologies are enabling new discoveries across different topics in translational and clinical science including viral host immune response and the tumor immune microenvironment. This virtual conference brings together research professionals, scientists, and clinicians around Asia Pacific to learn about and discuss recent discoveries in spatial biology.
 
Date: December 10, 2020
Time: 11-4pm, Korea
 
Event Highlight
  • Keynote lecture by Professor. Alex Swarbrick, Garvan Institute, Australia
  • More than 10 speakers from renowned institutes across Asia Pacific
  • An event co-organized by NanoString and Illumina
Invited Speakers
Arutha Kulasinghe
Queensland University of Technology
Alex Swarbrick
Garvan Institute of Medical Research
Quan Nguyen
University of Queensland
Subhra Biswas
Singapore Immunology Network
Ruby Huang
National Taiwan University
Nicholas West
Griffith University
Nan Wang
FynnBio
Patrick Danaher
NanoString Technologies

2. NEB 신제품 High Molecular Weight (HMW) DNA Extraction Kit로 megabase의 DNA를 빠르게 추출

Faster workflows, improved yields and quality for cells, blood, tissue & bacteria

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New products:
Monarch® HMW DNA Extraction Kits

Monarch HMW DNA Extraction Kit는 새로운 glass bead 기반 처리방식을 사용하여 사용자가 세포, 혈액, 조직 및 박테리아에서 동급 최고의 수율과 순도로 DNA를 빠르고 쉽게 Megabase(Mb) 범위로 추출 할 수 있습니다. 추출된 HMW DNA는 쉽게 용해되어 같은 날 사용할 수 있어서 추출 과정을 크게 단축 할 수 있습니다.

Highlights:

  • Fast workflows (cells: 30 minutes, blood: 60 minutes, tissue & bacteria: 90 minutes)
  • Extract DNA into the megabase (Mb) range from cells, blood, soft organ tissues, and bacteria
  • Tune DNA size based on agitation speed during lysis
  • Reproducibly achieve excellent yields and purity
  • Excellent performance in long read sequencing
See how our Cells & Blood Kit works in this short animation

Workflow for extraction from cells

Use of varying agitation speeds during lysis produces tunable fragment length of extracted HMW genomic DNA from cells and blood

Preps were performed on duplicate aliquots of 1 x 106 HEK 293 cells and 500 µl fresh human blood. Samples were agitated at the indicated speed during the lysis step to control the fragmentation of the DNA. Equal amounts of DNA from the replicates (cells: 500 ng; blood: 650 ng) were resolved by PFGE (1% agarose gel, 6 V/cm, 13°C for 20 hours, switch times ramped from 0.5–94 seconds on a BioRad CHEF-DR III System). Yield and purity ratios of the individual preps are shown in the accompanying tables. Lambda PFG Ladder and Lambda DNA-Hind III Digest (NEB #N0341 and #N3012) were used as molecular weight standards. Yield, purity ratios and DINs of the individual preps are shown in the accompanying tables.


We’ve had great success with obtaining HMW DNA for long read sequencing from a variety of cell types, using less input and obtaining a comparable yield… It is straightforward and easy to use. 

– Inswasti Cahyani & Matt Loose, DeepSeq, University of Nottingham

Extracted DNA from HEK293, human blood and mouse kidney samples generates excellent sequencing results on the Oxford Nanopore Technologies® platform
  HEK293 Sample 1 HEK293 Sample 2 Human Blood
Sample 1
Human Blood
Sample 2
Mouse Kidney
Sample
Mean read length (bases) 21338.9 19249.9 21522.6 24677.7 27120.7
Mean read quality 12.8 13.2 13.4 13.3 13
Median read length (bases) 10388 9702 10130 12593 23150
Median read quality 13.2 13.7 13.9 13.8 13.5
Number of reads 377687 633636 538090 327314 164000
Read length N50 (bases) 45432 40415 46542 51394 44631
Total bases 8.1 Gb 12.2 Gb 11.6 Gb 8.1 Gb 4.4 Gb

 Catalog #  Description  Size  Price
 T3050S  Monarch® HMW DNA Extraction Kit for Cells & Blood  5 prep  101,000
 T3050L  Monarch® HMW DNA Extraction Kit for Cells & Blood  50 prep  575,000
 T3060S  Monarch® HMW DNA Extraction Kit for Tissue  5 prep  108,000
 T3060L  Monarch® HMW DNA Extraction Kit for Tissue  50 prep  647,000
제품 문의


            

One or more of these products are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please contact NEB’s Global Business Development team at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

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Oxford Nanopore Technologies® is a registered trademark of Oxford Nanopore Technologies Ltd.

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3. SplintR Ligase를 이용한 Sensitive & Specific miRNA 검출법!

 

제품문의 ▷ https://bit.ly/38XC8o1

4. 실험 준비가 간편한 저산소 세포배양 키트

제품문의 ▷ https://bit.ly/38XC8o1

5. Novogene NGS Service를 경험하다!!!

제품문의 ▷ https://bit.ly/38XC8o1

            

 

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