BioWave,  Vol. 25 No. 6
연구실 홈페이지

 Our group focuses on devising convenient, sensitive, and reliable strategies for the detection of biomolecules and, in particular, on developing nanostructure-based methods including nanozymes. Current research also includes diverse biomedical studies including drug delivery, anti-bacterial assay, and biosynthesis of nanoparticles.


   PROF. MOON IL KIM / Department of BioNano Technology, Gachon University

   [1997.03 - 2001.02 ] Bachelor of Science, Department of Chemical and Biomolecular Engineering,
   Korea Advanced Institute of Science and Technology (KAIST)
   [2001.03 - 2002.08 ] Master of Science, Department of Chemical and Biomolecular Engineering,
   Korea Advanced Institute of Science and Technology (KAIST)
   [2002.09 - 2006.08 ] Doctor of Philosophy, Department of Chemical and Biomolecular Engineering,
   Korea Advanced Institute of Science and Technology (KAIST)
   [2006.09 - 2008.08 ] Postdoctoral Research Scientist, Department of Chemical and Biological Engineering,
   Rensselaer Polytechnic Institute (RPI), USA
   [2008.08 - 2010.05 ] Postdoctoral Research Scientist, Department of Chemical and Biomolecular Engineering,
   Korea Advanced Institute of Science and Technology (KAIST)
   [2010.06 - 2014.02 ] Research Assistant Professor, Department of Chemical and Biomolecular Engineering,
   Korea Advanced Institute of Science and Technology (KAIST)
   [2014.03 - 2019.09 ] Assistant Professor, Department of BioNano Technology, College of BioNano Technology, Gachon University
   [2019.10 - Present ] Associate Professor, Department of BioNano Technology, College of BioNano Technology, Gachon University
   [2022.02 - 2023.01 ] Visiting Professor, Department of Biomedical Engineering, University of North Carolina at Chapel Hill, USA








Researches regarding nanozyme (nanomaterial-based artificial enzyme) are emerging nowadays. Based on the enzyme-mimicking activity of nanozymes, we are developing novel biosensing platform technology. First, we have developed a new and simple colorimetric strategy for the detection of nucleic acids, which relies on target DNA-induced shielding action against the peroxidase mimicking activity of magnetic nanoparticles (MNPs). Second, we have developed a nanostructured multi-catalyst system consisting of MNPs as peroxidase mimetics and oxidative enzyme entrapped in large pore sized mesoporous silica. The nanocomposite concept was successfully demonstrated by very conveniently detecting target glucose or cholesterol revealing excellent reusability and stability. Besides, we developed highly efficient electrochemical biosensing system by employing conductive nanocomposite and ultrafast colorimetric immunoassay system by employing a nanocomposite entrapping magnetic and platinum nanoparticles. Finally, we are developing colorimetric biosensors based on new peroxidase mimetics including Fe-aminoclay and Cu-nanoflower. These achievements should accelerate and widen the utility of nanomaterial-based enzyme mimetics as next-generation alternatives to natural enzymes.



We have developed a solid-phase multiplexed amino acid array, which comprises 16 different amino acid E. coli auxotrophs yielding rapid, specific, and sensitive cell growth as a direct response to the concentration of corresponding amino acids. To obtain the resulting cell-based signal, bioluminescence or fluorescence-based measurement of cell density was employed. As a result, amino acid concentration-dependent response was produced. Such an approach is extending to rapid cell-based sensing of homocysteine and galactose that are relevant to human disease.





Nanozymes have also been extensively employed for the colorimetric detection of fungal toxins. Lai et al. developed a nanozyme-mediated strategy to visually detect AFB1. The method was based on the dissolution of MnO2 nanoflakes, which originally showed peroxidase-like activity, into Mn2+ in the presence of ascorbic acid, leading to the loss of activity. Specifically, free AFB1 in the sample and immobilized AFB1 on BSA-modified magnetic beads (AFB1-MB) were competitive antigens for the AFB1 antibody, which was immobilized on ascorbate oxidase-conjugated AuNPs (Ab-AuNPs-AOx). As the concentration of AFB1 in the sample increased, the amount of conjugate between AFB1-MB and Ab-AuNPs-AOx decreased, resulting in less AOx captured by magnetic separation. With less-captured AOx, the degradation of ascorbic acid was hindered and a relatively higher concentration of ascorbic acid remained, leading to the effective dissolution of MnO2 nanoflakes, thus inhibiting their peroxidase activity.


최근 3년, 2020~2022

- 2022
Quynh Huong Nguyen, Dong Hoon Lee, Phuong Thy Nguyen, Phan Gia Le and Moon Il Kim*, “Foldable paper microfluidic device based on single iron site-containing hydrogel nanozyme for efficient glucose biosensing”, Chemical Engineering Journal 454, 140541 (2023.02.15) (ISSN: 1385-8947, IF: 16.744)

Phan Gia Le, Qilu Wu, Do Yun Kong, Jun Ge* and Moon Il Kim*, “Tailoring nanostructured supports to achieve high performance in enzymatic biofuel cells”, ACS Applied Energy Materials 5(11), 13113-13127 (2022.11.28) (ISSN: 2574-0962, IF: 6.959)

Trung Hieu Vu, Phuong Thy Nguyen, Moon Il Kim*, “Polydopamine-coated Co3O4 nanoparticles as an efficient catalase mimic for fluorescent detection of sulfide ion” Biosensors 12, 1047 (2022.11.19) (ISSN: 2079-6374, IF: 5.743)

Yejin Park, Thinh Viet Dang, Uiseok Jeong, Moon Il Kim*, Jinsik Kim*, “Comparison of optical and electrical sensor characteristics for efficient analysis of attachment and detachment of aptamer”, Biosensors 12, 979 (2022.11.07) (ISSN: 2079-6374, IF: 5.743)

Bumjun Park, Thinh Viet Dang, Jingon Yoo, Tai Duc Tran, Seyed Majid Ghoreishian, Gyu Heun Lee, Moon Il Kim* and Yun Suk Huh*, "Silver nanoparticle-coated polydopamine-copper hybrid nanoflowers as ultrasensitive surface-enhanced Raman spectroscopy probes for detecting thiol-containing molecules", Sensors and Actuators B: Chemical 369, 132246 (2022.10.15)(ISSN:0925-4005, IF:9.221)

Thao Nguyen Le, Xuan Ai Le, Tai Duc Tran, Kang Jin Lee, and Moon Il Kim*, "Mn-Cu nanoflowers as efficient laccase mimics for on-site visual detection of phenolic neurotransmitters in paper microfluidic device and rapid degradation of dyes", Journal of Nanobiotechnology 20,358 (2022.08.02)(ISSN: 1477-3155, IF: 9.429)

Jeong Un Kim, Kang Jin Lee and Moon Il Kim*, "Characteristics of Prussian blue nanoparticles and trends in their biotechnological application research (프러시안블루 나노입자의 특성과 생물공학적 응용연구 동향)", KSBB Journal 37(2). 41-48 (2022.06.30)(ISSN: 1225-7117, IF: Not determined)

Phuong Thy Nguyen, Junsang Lee, Ara Cho, Min Su Kim, Daeeun Choi, Jeong Woo Han,* Moon Il Kim* and Jinwoo Lee*, "Rational development of Co-doped mesoporous ceria with high peroxidase -mimicking activity at neutral pH for paper-based colorimetric detection of multiple biomarkers", Advanced Functional Materials 32(21), 2112428 (2022.05.19) (ISSN: 1616-301X, IF: 18.808)

Do Yun Kong, Nam Su Heo, Ji Won Kang, Jin Bae Lee, Hae Jin Kim* and Moon Il Kim*, “Nanoceria-based lateral flow immunoassay for hydrogen peroxide-free colorimetric biosensing for C-reactive protein”, Analytical and Bioanalytical Chemistry 414(10), 3257-3265 (2022.04.01) (ISSN: 1618-2642, IF: 4.157)

Weldejewergis Gebrewahid Kidanu, Jaehyun Hur, Hyung Wook Choi, Moon Il Kim* and Il Tae Kim*, “High capacity and inexpensive multivalent cathode materials for aqueous rechargeable Zn-ion battery fabricated via in situ electrochemical oxidation of VO2 nanorods”, Journal of Power Sources 523, 231060 (2022.03.01) (ISSN: 0378-7753, IF: 9.127)

Ho Kyeong Kim, Phuong Thy Nguyen, Moon Il Kim* and Byoung Chan Kim*, “Aptamer-functionalized and silver-coated polydopamine-copper hybrid nanoflower adsorbent embedded with magnetic nanoparticles for efficient mercury removal”, Chemosphere 288, 132583 (2022.02.15) (ISSN: 0045-6535, IF: 7.086)

Xuan Ai Le, Thao Nguyen Le and Moon Il Kim*, “Dual-functional peroxidase-copper phosphate hybrid nanoflowers for sensitive detection of biological thiols”, International Journal of Molecular Sciences 23(1), 366 (2022.01.10) (ISSN: 1422-0067, IF: 5.924)


- 2021
Kang Jin Lee, Phan Gia Le and Moon Il Kim*, “Recent advances in research on implantable enzymatic biofuel cell (생체 이식형 효소 기반 바이오 연료전지 연구의 최근 동향)”, KSBB Journal 36(4), 238-246 (2021.12.31) (ISSN: 1225-7117, IF: Not determined)

Bhaskar Das, Javier Lou Franco, Natasha Logan, Paramasivan Balasubramanian, Moon Il Kim and Cuong Cao*, “Nanozymes in point-of-care diagnosis: An emerging futuristic approach for biosensing” Nano-Micro Letters 13, 193 (2021.12.15) (ISSN: 2311-6706, IF: 16.419)

Thinh Viet Dang, Nam Su Heo, Hye-Jin Cho, Sang Moon Lee, Min Young Song, Hae Jin Kim* and Moon Il Kim*, “Colorimetric determination of phenolic compounds using peroxidase mimics based on biomolecule-free hybrid nanoflowers consisting of graphitic carbon nitride and copper”, Microchimica Acta 188, 293 (2021.09.15) (ISSN: 0026-3672, IF: 5.833)

Phan Gia Le and Moon Il Kim*, “Research progress and prospects of nanozyme-based glucose biofuel cells”, Nanomaterials 11(8), 2116 (2021.08.19) (ISSN: 2079-4991, IF: 5.076)

Moon Il Kim, Chan Yeong Park, Ji Min Seo, Kyoung Suk Kang, Ki Soo Park, Jongeun Kang, Kwan Soo Hong, Yoojin Choi, Sang Yup Lee, Jong Pil Park, Hyun Gyu Park* and Tae Jung Park*, “In situ biosynthesis of a metal nanoparticle encapsulated in alginate gel for imageable drug-delivery system”, ACS Applied Materials and Interfaces 13, 31, 36697-36708 (2021.08.11) (ISSN: 1944-8244, IF: 9.229) Highlighted as an inside cover article

Yoon Jung Jang, Vu Khac Hoang Bui, Phuong Thy Nguyen, Young-Chul Lee* and Moon Il Kim*, “UV-light-driven enhancement of peroxidase-like activity of Mg-aminoclay-based Fe3O4/TiO2 hybrids for colorimetric detection of phenolic compounds”, Chemosensors 9(8), 219 (2021.08.11) (ISSN: 2227-9040, IF: 3.398)

Hee Tae Ahn, In Seung Jang, Thinh Viet Dang, Yi Hyang Kim, Dong Hoon Lee, Hyeon Seok Choi, Byung Jo Yu* and Moon Il Kim*, “Effective cryopreservation of a bioluminescent auxotrophic Escherichia coli-based amino acid array to enable long-term ready-to-use applications”, Biosensors 11, 252 (2021.07.26) (ISSN: 2079-6374, IF: 5.519)

Hoang Van Quy, Trung Hieu Vu, Dang Hai Truyen, Moon Il Kim and Chung Wung Bark*, “Investigation of the influence of TiO2 distribution on HA/TiO2 composite wetting ability using the dispersant SDBS, high-temperature annealing, and ultrasonication”, Biomedical Materials 16(4), 045033 (2021.07.15) (ISSN: 1748-6041, IF: 3.174)

Zakir Ullah, Kang Kim, Arramshetti Venkanna, Hye Su Kim, Moon Il Kim and Mi-hyun Kim*, “Plausible pnicogen bonding of epi-Cinchonidine as a chiral scaffold in catalysis”, Frontiers in Chemistry 9, 669515 (2021.07.06) (ISSN: 2296-2646, IF: 5.221)

Tai Duc Tran, Phuong Thy Nguyen, Thao Nguyen Le, and Moon Il Kim*. "DNA-copper hybrid nanoflowers as efficient laccase mimics for colorimetric detection of phenolic compounds in paper microfluidic devices", Biosensors and Bioelectronics 182, 113187 (2021.06.15) (ISSN: 0956-5663, IF: 10.257)

Quynh Huong Nguyen and Moon Il Kim*, "Using nanomaterials in colorimetric toxin detection", BioChip Jounal, 1-12 (2021.06.15) (ISSN: 1976-0280, IF: 2.309)

Hong Jae Cheon, Quynh Huong Nguyen and Moon Il Kim*, "Highly sensitive fluorescent detection of acetylcholine based on the enhanced peroxidase-like activity of histidine coated magnetic nanoparticles", Nanomaterials 11, 1207 (2021.05.01) (ISSN: 2079-4991, IF: 4.324)


- 2020
Thuan Ngoc Vo, Tai Duc Tran, Hoang Kha Nguyen, Do Yun Kong, Moon Il Kim*, Il Tae Kim*, “In situ growth of hybrid nanoflowers on activated carbon fibers as electrodes for mediatorless enzymatic biofuel cells”, Materials Letters 281, 128662 (2020.12.15) (ISSN: 0167-577X, IF: 3.204)

Quynh Huong Nguyen and Moon Il Kim*, “Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection”, TrAC-Trends in Analytical Chemistry 132, 116038 (2020.11.15) (ISSN: 0165-9936, IF: 9.801)

Hee Tae Ahn, Ha-Rim An, Yong Cheol Hong, Soon Chang Lee, Thao Nguyen Le, Xuan Ai Le, Ho Seung Kwak, Young-Seak Lee, Yesul Jeong, Ji-In Park, Hyeran Kim, Moonsang Lee, Seung Jo Yoo, Sang-Gil Lee, Kyuseok Choi, Young-Boo Lee, Moon Il Kim* and Hyun Uk Lee*, “Ultrarapid, size-controlled, high-crystalline plasma-mediated synthesis of ceria nanoparticles for reagent-free colorimetric glucose test strips”, Sensors and Actuators B: Chemical 320, 128404 (2020.10.15) (ISSN: 0925-4005, IF: 6.393)

Kumud Malika Tripathi, Hee Tae Ahn, Minsoo Chung, Xuan Ai Le, Deepika Saini, Anshu Bhati, Sumit Kumar Sonkar, Moon Il Kim* and TaeYoung Kim*, “N, S, and P-co-doped carbon quantum dots: Intrinsic peroxidase activity in a wide pH range and its antibacterial applications” , ACS Biomaterials Science and Engineering 6(10), 5527-5537 (2020.10.12) (ISSN: 2373-9878, IF: 4.152)

Hye Su Kim, Ji Sun Lee and Moon Il Kim*, “Poly-γ-glutamic acid/chitosan hydrogel nanoparticles entrapping glucose oxidase and magnetic nanoparticles for glucose biosensing”, Journal of Nanoscience and Nanotechnology 20(9), 5333-5337 (2020.09.15) (ISSN: 1533-4880, IF: 1.093)

Phuong Thy Nguyen, Young Im Kim and Moon Il Kim*, “Reagent-free colorimetric cholesterol test strip based on self color-changing property of nanoceria”, Frontiers in Chemistry 8, 798 (2020.09.02) (ISSN: 2296-2646, IF: 3.693)

Inseon Lee, Hong Jae Cheon, Manab Deb Adhikari, Tai Duc Tran, Kyung-Min Yeon, Moon Il Kim*, and Jungbae Kim*, “Glucose oxidase-copper hybrid nanoflowers embedded with magnetic nanoparticles as an effective antibacterial agent”, International Journal of Biological Macromolecules 155, 1520-1531 (2020.07.01) (ISSN: 0141-8130, IF: 4.784)

Nam Su Heo, Han Pill Song, Sang Moon Lee, Hye-Jin Cho, Hae Jin Kim, Yun Suk Huh*, and Moon Il Kim*, “Rosette-shaped graphitic carbon nitride acts as a peroxidase mimic in a wide pH range for fluorescence-based determination of glucose with glucose oxidase”, Microchimica Acta 187: 286 (2020.05.15) (ISSN: 0026-3672, IF: 5.479)

Phuong Thy Nguyen, Hee Tae Ahn and Moon Il Kim*, “Reagent-free colorimetric assay for galactose using agarose gel entrapping nanoceria and galactose oxidase”, Nanomaterials 10, 895 (2020.05.08) (ISSN: 2079-4991, IF: 4.034)

Sujeong Shin†, Han Sol Kim†, Moon Il Kim†, Jinwoo Lee, Hyun Gyu Park* and Jungbae Kim*, “Crowding and confinement effects on enzyme stability in mesoporous silicas”, International Journal of Biological Macromolecules 144, 118-126 (2020.02.01) (ISSN: 0141-8130, IF: 4.784)

Min Su Kim, Do Hyun Kim, Junsang Lee, Hee Tae Ahn, Moon Il Kim* and Jinwoo Lee*, “Self color-changing ordered mesoporous ceria for reagent-free colorimetric biosensing”, Nanoscale 12(3), 1419-1424 (2020.01.21) (ISSN: 2040-3364, IF: 6.970)

Min Su Kim, Junsang Lee, Hye Su Kim, Ara Cho, Kyu Hwan Shim, Thao Nguyen Le, Seong Soo A. An, Jeong Woo Han*, Moon Il Kim* and Jinwoo Lee*, “Heme cofactor-resembling Fe-N single site embedded graphene as nanozymes to selectively detect H2O2 with high sensitivity”, Advanced Functional Materials 30(1), 1905410 (2020.01.03) (ISSN: 1616-301X, IF: 15.621)

Thao Nguyen Le, Tai Duc Tran and Moon Il Kim*, “A convenient colorimetric bacteria detection method utilizing chitosan-coated magnetic nanoparticles”, Nanomaterials 10, 92 (2020.01.02) (ISSN: 2079-4991, IF: 4.034)



 지도교수: 김문일 교수

   (2022년 현재)
 Research Professor : Phan Gia Le
 PhD. Course : Nguyen Ngoc Phuong Thy, Vu Trung Hieu, Dang Viet Thinh
 MS. Course : Chau Phan Ba Khanh, Kang jin Lee, Kim Jeong Un, Kim Jee Min,
                         Kim Kyung Mi, Kwak Kyung Min
 Contact : , +82-31-750-8563 (Office)

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등록일 2023.01.02
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