BioWave,  Vol. 25 No. 1
경희대학교 화학공학과 NANOBIO ENGINEERING LAB 이은열 교수
연구실 홈페이지
Introduction  
 

 본 연구실에서는 바이오인포메틱스, 나노바이오촉매와 대사공학 기반으로 Functional Biomaterials 생합성 기술 및 Cell Factory Platform 기술을 개발 중이며, 나노-바이오전환 공정 개발을 통해 의약품 및 기능성 화장품 바이오소재, 친환경 화학소재 등을 제조하는 원천핵심 바이오기술을 개발하고 있습니다. 탄소중립, 지구 온난화 및 기후변화 대응 관련하여 바이오매스와 메탄, 이산화탄소 등과 같은 온실가스를 차세대 탄소자원으로 활용하여 생분해성 바이오플라스틱, 바이오에너지 등을 제조하는 탄소 중립형 C1 바이오리파이너리 기술개발 등을 수행하고 있습니다.

 Prof. Eun Yeol LEE obtained his bachelor in 1989 from Seoul National University. He got his Ph.D. in 1995 from Seoul National University. He worked at Microbiology Institute of Goettingen University (Germany), Industrial Microbiology and Food Biotechnology Institute of Wageningen University (The Netherlands) and Biomedical Engineering Department at Cornell University (USA) as a visiting scholar. He was a senior researcher at Samsung Advanced Institute of Science and Technology, and then an associate professor of Department of Food Science and Biotechnology at Kyungsung University. Currently, he is the Kyung Hee University Fellow and professor of Chemical Engineering Department. His research interests are Bioinformatics-inspired Protein/Metabolic Engineering, Nanobiocatalysis, and Biomanufacturing of Functional Biomaterials for pharmaceuticals and cosmetics, Biofuels, Biochemicals and Biopolymers from lignocellulosic biomass, macro-/microalgae, methane and carbon dioxide.

 
Research  
 

 1. 연구 분야

 - 효소 및 발효 바이오전환 기술을 이용한 의약품, 기능성 화장품 소재 개발
 - 미세조류 및 해조류 등 바이오매스 유래 유용성분을 활용한 기능성 바이오 소재 개발
 - 리그닌 등을 활용한 IT 소재 및 자동차 내장재용 바이오플라스틱 소재 개발
 - 셰일가스 메탄, 바이오가스, 이산화탄소 등 C1 바이오전환 기술을 이용한 기능성 바이오소재, 바이오플라스틱, 화학소재 및 바이오에너지 제조
 - 메탄자화균 플랫폼 균주 개발 및 이를 활용한 온실가스로부터 생분해성 바이오플라스틱 제조 및 기능성 Feed 제조 기술개발

 2. RESEARCH INTERESTS

 - Biosynthesis of pharmaceutical and cosmetics biomaterials using enzyme and fermentation technology
 - Development of functional biomaterials through biosynthesis of natural products derived from biomass including microalgae and seaweed
 - Lignin-based bioplastic materials for IT materials and automotive interior materials
 - Biomanufacturing of functional biomaterials, bioplastics, chemicals, and biofuels using C1 bioconversion technology for methane & carbon dioxide
 - Methanotrophic cell factory platform for biodegradable bioplastics and functional feed from methane and carbon dioxide

 
Publications  
 

[ 2022 年 ]

258. Diep Ngoc Pham, Anh Duc Nguyen, Eun Yeol Lee (2022) Outlook on engineering biocatalysts for one-carbon-based industrial biotechnology. Chemical Engineering Journal. In revision. [IF 13.273, 2.8%]

257. Diep Ngoc Pham, Anh Duc Nguyen, So Hyeon Oh, and Eun Yeol Lee (2022) Bypassing the bottlenecks in the shikimate and methylerythritol phosphate pathways for enhancing the production of natural products from methane in Methylotuvimicrobium alcaliphilum 20Z. Green Chem. 2022, 24, 2893 - 2903 https://doi.org/10.1039/D2GC00077F [IF 10.182, JCR 4.5%] Corresponding author

256. Su Yeon Bak, Seung Gi Kang, Kyu Hwan Choi, Ye Rim Park, Eun Yeol Lee, and Bum Jun Park (2022) Phase-transfer biocatalytic methane-to-methanol conversion using the spontaneous phase-separable membrane μCSTR. J. Ind. Eng. Chem. https://doi.org/10.1016/j.jiec.2022.04.021

255. Arslan Sarwar, Linh Thanh Nguyen, Eun Yeol Lee (2022) Bio-upgrading of ethanol to fatty acid ethyl esters by metabolic engineering of Pseudomonas putida KT2440. Bioresource Tech. 350, 126899 . https://doi.org/10.1016/j.biortech.2022.126899 [IF 9.642, 7.1%, April 2022 ] Corresponding author

254. Yerin Lee, My Ha Tran and Eun Yeol Lee (2022) Hydroxymethylation of technical lignins obtained from different pretreatments for preparation of high performance biopolyurethane foam. European Journal of Wood and Wood Products. https://doi.org/10.1007/s00107-022-01845-z [IF 2.014, JCR 31.8%] Corresponding author

253. Hoa Thi Quynh Le, Dung Hoang Anh Mai , Eun Yeol Lee (2022) Development of Methylorubrum extorquens AM1 as a Promising Platform Strain for Enhanced Violacein Production via Co-Utilization of Methanol and Acetate. Metab. Eng. 72, 150-160. https://doi.org/10.1016/j.ymben.2022.03.008 [IF 9.783, JCR 6.3%; July 2022] Corresponding author

252. Guo S., Nguyen D.T.N., Chau T.H.T., Fei Q., Lee E.Y. (2022) Systems Metabolic Engineering of Methanotrophic Bacteria for Biological Conversion of Methane to Value-Added Compounds. In: . Advances in Biochemical Engineering/Biotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2021_184

251. Tin Hoang Trung Chau, Anh Duc Nguyen and Eun Yeol Lee (2022) Enabling the acetol production in engineered methanotrophic biocatalyst Methylomonas sp. DH-1 by the coupling activity of heteroexpressed novel protein PmoD with endogenous particulate methane monooxygenase. Biotech. Biofuels. 15, 7. https://doi.org/10.1186/s13068-022-02105-1 [IF 6.040, JCR 12.6%] Corresponding author

250. Hye-Jin Jo, Jun-Hong Kim, Ye-Na Kim, Pil-Won Seo, Chae-Yun Kim, Ji-Won Kim, Han-Na Yu, Huijin Cheon, Eun Yeol Lee, Jeong-Sun Kim, and Jin-Byung Park (2022) Glyoxylate Carboligase-based Whole-Cell Biotransformation of Formaldehyde into Ethylene Glycol via Glycolaldehyde. Green Chem. 24, 218-226. DOI: https://doi.org/10.1039/D1GC03549E [IF 10.182, TOP 4.5%]

249. Diep Ngoc Pham, Dung Hoang Anh Mai, Anh Duc Nguyen, Tin Hoang Trung Chau, Eun Yeol Lee (2022) Development of an engineered methanotroph-based microbial platform for biocatalytic conversion of methane to phytohormone for sustainable agriculture. Chemical Engineering Journal. 429, 132522. https://doi.org/10.1016/j.cej.2021.132522. [IF 13.273, 2.8%]

[2021 年]

248. Eun Yeol Lee, Fu-Li Li, Yu Wang, Pramod P. Wangikar, Michael Thomas Guarnieri, Guodong Luan (2021) Editorial: Bioconversion and Biorefinery of C1 Compounds. Front. Microbiol. 12:778962 doi: 10.3389/fmicb.2021.778962 [IF 5.640]

247. Dung Hoang Anh Mai, Thu Thi Nguyen , and Eun Yeol Lee (2021) The Ethylmalonyl-CoA Pathway for Methane-based Biorefineries: A Case Study of Using Methylosinus trichosporium OB3b, an Alpha-proteobacterial Methanotroph, for Producing 2-Hydroxyisobutyric Acid and 1,3-Butanediol from Methane. Green Chem. 23, 7712 - 7723. https://doi.org/10.1039/D1GC02866A [IF 10.182, 4.5%]

246. My Ha Tran, Dieu-Phuong Phan, Thuy Ha Nguyen, Han Bom Kim, Jinsoo Kim, Eun Duck Park, Eun Yeol Lee (2021) Catalytic hydrogenolysis of alkali lignin in supercritical ethanol over copper monometallic catalyst supported on a chromium-based metal?organic framework for the efficient production of aromatic monomers. Bioresource Technol. 342, 125941 [IF 9.642, 7.1%]

245. Hye Soo Lee, Hong-Ju Lee, Sang Hyun Kim, Jang Yeon Cho, Min Ju Suh, Sion Ham, Shashi Kant Bhatia, Ranjit Gurav, Yun-Gon Kim, Eun Yeol Lee, Yung-Hun Yang (2021) Novel phasins from the Arctic Pseudomonas sp. B14-6 enhance the production of polyhydroxybutyrate and increase inhibitor tolerance. International Journal of Biological Macromolecules. 190, 722-729. DOI: https://doi.org/10.1016/j.ijbiomac.2021.08.236 [IF 6.953, JCR Top 6.8%]

244. Dieu-Phuong Phan, Van Nhieu Le, Jinsoo Kim, Eun Yeol Lee (2021) Controlled hydrodeoxygenation of lignin-derived anisole over supported Pt on Zr-based MOFs through defect engineering approach. Fuel Processing Technology. 224, 107001. [IF 7.033]

243. Linh Thanh Nguyen, My Ha Tran, Eun Yeol Lee (2021) Co-upgrading of ethanol-assisted depolymerized lignin: a new biological lignin valorization approach for the production of protocatechuic acid and polyhydroxyalkanoic acid. Bioresource Tech, 338, 125563. [IF 9.642, 7.1%]

242. Anh Duc Nguyen, Diep Ngoc Pham, Tin Hoang Trung Chau, Eun Yeol Lee (2021) Enhancing sesquiterpenoids production from methane via synergy of methylerythritol phosphate pathway and a short-cut route to 1-deoxy-D-xylulose 5-phosphate in methanotrophic bacteria. Microorganisms 2021, 9, 1236. [IF 4.152] [7 June 2021] https://doi.org/10.3390/microorganisms9061236

241. My Ha Tran, Dieu-Phuong Phan and Eun Yeol Lee (2021) Review on lignin modifications toward natural UV protection ingredient for lignin-based sunscreens. Green Chem. 23, 4633 - 4646 https://doi.org/10.1039/D1GC01139A. [IF 10.182, 4.5%]

240. Thu Thi Nguyen, Eun Yeol Lee (2021) Methane-based biosynthesis of 4-hydroxybutyrate and P(3-hydroxybutyrate-co-4-hydroxybutyrate) using engineered Methylosinus trichosporium OB3b. Bioresource Tech, 335, 125263. https://doi.org/10.1016/j.biortech.2021.125263. [IF 9.642, 7.1%] [1 September 2021]

239. Dieu-Phuong Phan; Van Nhieu Le; Thuy Ha Nguyen; Han Bom Kim; Eun Duck Park; Jinsoo Kim; Eun Yeol Lee (2021) Effect of amino-defective-MOF materials on the selective hydrodeoxygenation of fatty acid over Pt-based catalysts. J. Catal. 400, 283-293. [August 2021] [IF 7.920, 10.4%]

238. My Ha Tran, Ju-Hyun Yu, and Eun Yeol Lee (2021) Microwave-assisted two-step liquefaction of acetone-soluble lignin of silvergrass saccharification residue for production of biopolyol and biopolyurethane. Polymers, 13, 1491. [IF 3.426]

237. Sun Mi Lee; Hong-Ju Lee; Sang Hyun Kim; Min Ju Suh; Jang Yeon Cho; Sion Ham; Jong-Min Jeon; Jeong-Jun Yoon; Shashi Kant Bhatia; Ranjit Gurav; Eun Yeol Lee; Yung-Hun Yang (2021) Screening of the strictly xylose-utilizing Bacillus sp. SM01 for polyhydroxybutyrate and its co-culture with Cupriavidus necator NCIMB 11599 for enhanced production of PHB. International Journal of Biological Macromolecules.181, 410-417. [IF 6.953, JCR Top 6.8%]

236. Hoa Thi Quynh Le, Anh Duc Nguyen, Ye Rim Park, Eun Yeol Lee (2021) Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi-carbon utilizing pathway in obligate methanotrophic bacteria. Microb Biotechnol DOI:10.1111/1751-7915.13809. [IF 5.813]

235. Mrudulakumari Vasudevan Ushasree, Ok Kyung Lee, Eun Yeol Lee (2021) Alginate derived functional oligosaccharides: Recent developments, barriers, and future outlooks. Carbohydrate Polymers, 267, 118158. https://doi.org/10.1016/j.carbpol.2021.118158. [IF 9.381, JCR 3.4%] [1 September 2021]

234. Ye Rim Park, Dong Ho Kim, Kyu Hwan Choi, Yong Woo Kim, Eun Yeol Lee, Bum Jun Park (2021) Biofuel upgrade reactions via phase-transfer catalysis of methanotrophs. J. Ind. Eng. Chem. 95, 305-311. [25 March 2021] https://doi.org/10.1016/j.jiec.2021.01.007. [IF 5.278]

233. Anh Duc Nguyen, Tin Hoang Trung Chau, Eun Yeol Lee (2021) Methanotrophic microbial cell factory platform for simultaneous conversion of methane and xylose to value-added chemicals. Chemical Engineering Journal. 420 (2), 127632. DOI: 10.1016/j.cej.2020.127632 [Available online 5 November 2020] [IF 13.273, JCR 2.8%]

232. Anh Duc Nguyen, Eun Yeol Lee (2021) Engineered methanotrophy: a sustainable solution for methane-based industrial biomanufacturing. Trends in Biotechnology, 39(4), 381-396 doi: 10.1016/j.tibtech.2020.07.007 [April 2021]. [IF 19.536, JCR 1.9%]

231. Yerin Lee, My Ha Tran and Eun Yeol Lee (2021) Acid-base catalyzed two-step liquefaction of empty fruit bunch (EFB) lignin residue for preparation of biopolyol and high-performance biopolyurethanes. Wood Sci. Technol. 55(2), 315-330. doi: 10.1007/s00226-021-01267-9 [17-02-2021] [IF 2.109, JCR 9.5%]

230. Linh Thanh Nguyen, Dieu-Phuong Phan, Arslan Sarwar, My Ha Tran, Ok Kyung Lee, Eun Yeol Lee (2021) Valorization of industrial lignin to value-added chemicals by chemical depolymerization and biological conversion. Ind. Crop. Prod.,161, 113219. DOI: 10.1016/j.indcrop.2020.113219 [17 March 2021] [IF 4.244, JCR 8.8%].

229. Diep Thi Ngoc Nguyen, Ok Kyung Lee, Nguyen Thi Thu and Eun Yeol Lee (2021) Type II methanotrophs: a promising microbial cell-factory platform for bioconversion of methane to chemicals. Biotechnol. Adv., 47, 107700. https://doi.org/10.1016/j.biotechadv.2021.107700 [1 March 2021] [IF 10.744, JCR 3.8%]

228. L. Ou, C. Dou, J-H Yu, H. Kim, Y-C Park, E.Y. Lee, S. Kelley, S. Park (2021) Techno-economic analysis of sugar production from lignocellulosic biomass with utilization of hemicellulose and lignin for high-value co-products. Biofuels, Bioproducts & Biorefining [IF 4.528 ] 15(2), 404-415. DOI: 10.1002/bbb.2170 [First online published: 19 November 2020].

[ 2020年 ]

227. Thu Thi Nguyen, Ok Kyung Lee, Sanzhar Naizabekov, Eun Yeol Lee (2020) Bioconversion of methane to lysine and cadaverine in engineered type II methanotroph, Methylosinus trichosporium OB3b. Green Chem [IF 9.480, JCR 4.9%] 22, 7803-7811. DOI: 10.1039/D0GC02232B. Featured as Back Cover Article. Selected as 2020 Green Chemistry Hot Article

226. Tin Hoang Trung Chau, Eun Yeol Lee (2020) Development of cell-free platform-based toehold switch system for detection of IP-10 mRNA, an indicator for acute kidney allograft rejection diagnosis. Clinica Chimica Acta 510, 619-624. [IF 2.615] DOI: 10.1016/j.cca.2020.08.034 [15 October 2020]

225. Anh Duc Nguyen, Gayoung Nam, Donghyuk Kim, Eun Yeol Lee (2020) Metabolic role of pyrophosphate-linked phosphofructokinase pfk for C1 assimilation in Methylotuvimicrobium alcaliphilum 20Z. Microbial Cell Factories [IF 4.187]. 19,131. DOI: 10.1186/s12934-020-01382-5 [16 June 2020]

224. Yoo Jung Chu, Hae Sol Kim, Myung Sun Kim, Eun Yeol Lee, Hee Sook Kim (2020) Functional characterization of a novel oligoalginate lyase of Stenotrophomonas maltophilia KJ-2 using site-specific mutation reveals bifunctional mode of action, possessing both endolytic and exolytic degradation activity toward alginate in seaweed biomass. Front. Mar. Sci. [IF 3.661, JCR 6.6%] 7, 420. DOI:10.3389/fmars.2020.00420. [03 June 2020]

223. Tin Hoang Trung Chau, Dung Hoang Anh Mai, Diep Ngoc Pham, Hoa Thi Quynh Le, Eun Yeol Lee (2020) Developments of riboswitches and toehold switches for molecular detection - biosensing and molecular diagnostics. Int. J. Mol. Sci. [IF 4.556] 21, 3192. [30 April 2020] doi: 10.3390/ijms21093192

222. Mrudulakumari Vaudevan Ushasree, Eun Yeol Lee (2020) Flavonoids, terpenoids, and polyketide antibiotics: Role of glycosylation and biocatalytic tactics in engineering glycosylation. Biotechnol. Adv. [IF 12.831, JCR 3.8%] 41, 107550. DOI: 10.1016/j.biotechadv.2020.107550. [Available online 1 May 2020, 107550]

221. Anh Duc Nguyen, Donghyuk Kim, Eun Yeol Lee (2020) Unlocking the biosynthesis of sesquiterpenoids from methane via the methylerythritol phosphate pathway in methanotrophic bacteria, using α-humulene as a model compound. Metab. Eng. [IF 7.263, JCR 8.3%] 61, 69-78. DOI: 10.1016/j.ymben.2020.04.011. [Available online 5 May 2020; September 2020]

220. Dieu-Phuong Phan, Eun Yeol Lee (2020) Phosphoric acid enhancement in a Pt-encapsulated Metal-Organic Framework (MOF) bifunctional catalyst for efficient hydro-deoxygenation of oleic acid from biomass. J. Catal. [IF 7.888, JCR 4.9%] 386, 19-29. [June 2020]

219. Sanzhar Naizabekov, Eun Yeol Lee (2020) Genome-scale metabolic model reconstruction and in silico investigations of methane metabolism in Methylosinus trichosporium OB3b. Microorganisms [IF 4.152] 2020, 8, 437. DOI:10.3390/microorganisms8030437. [27 March 2020]

218. Yoseb Song, Jin-Soo Lee, Jongoh Shin, Gyu Min Lee, Sangrak Jin, Seul-Gi Kang, Jung-Kul Lee, Dong Rip Kim, Eun Yeol Lee, Sun Chang Kim, Suhyung Cho, Donghyuk Kim and Byung-Kwan Cho (2020) Functional cooperation of the glycine synthase-reductase and Wood Ljungdahl pathways for autotrophic growth of Clostridium drakei. Proceedings of the National Academy of Sciences of the United States of America [IF 9.412] 117(13), 7516-7523. [31 March 2020] doi: 10.1073/pnas.1912289117

217. Diep Thi Ngoc Nguyen, Ok Kyung Lee, Chaeil Lim, Jinwon Lee, Jeong-Geol Na, and Eun Yeol Lee (2020) Metabolic engineering of type II methanotroph, Methylosinus trichosporium OB3b, for production of 3-hydroxypropionic acid from methane via a malonyl-CoA reductase-dependent pathway. Metab. Eng. [IF 7.263, JCR 8.3%] 59, 142-150. [May 2020]

216. Dieu-Phuong Phan, The Ky Vo, Van Nhieu Le, Jinsoo Kim, Eun Yeol Lee (2020) Spray pyrolysis synthesis of bimetallic NiMo/Al2O3-TiO2 catalyst for hydrodeoxygenation of guaiacol. J. Ind. Eng. Chem. [IF 5.278] 83, 351-358. [25 March 2020]

215. Pil-Won Seo, Hye-Jin Jo, In Yeub Hwang, Ha-Yeon Jeong, Jun-Hong Kim, Ji-Won Kim, Eun Yeol Lee, Jin-Byung Park, and Jeong-Sun Kim (2020) Understanding the molecular properties of the E1 subunit (SucA) of α-ketoglutarate dehydrogenase complex from Vibrio vulnificus for the enantioselective ligation of acetaldehydes into (R)-acetoin. Catalysis Science & Technology [IF 5.721, featured as Back Cover Article]. 10, 79-85.

214. Dieu-Phuong Phan and Eun Yeol Lee (2020) Controlled hydrogenolysis over heterogeneous catalysts for lignin valorization. Catalysis Reviews-Science and Engineering. [IF 11.389] 62, 607-630. DOI: 10.1080/01614940.2020.1770401. [Published online: 04 Jun 2020]

213. My Ha Tran and Eun Yeol Lee (2020) Development and optimization of solvothermal liquefaction of marine macroalgae Saccharina japonica biomass for biopolyol and biopolyurethane production. J. Ind. Eng. Chem. [IF 5.278] 81, 167-177.

212. Anh Duc Nguyen, Joon Young Park, In Yeub Hwang, Richard Hamilton, Marina G. Kalyuzhnaya, Donghyuk Kim, Eun Yeol Lee (2020) Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol. Metab. Eng. [IF 7.263, JCR 8.3%] 57, 1-12. [ January 2020]

 

 
     
 
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 지도교수: 이은열 교수

   (2022년 현재)
 Research Professor: Dr. Ok Kyung Lee, Dr. Shyam Krishna
 Post-doctoral Researcher: Dr. Ushasree M. V, Dr. Dieu-Phuong Phan, Dr. Linh Nguyen Thanh
 Graduate Students: Tin Chau Hoang Trung, Dung Mai Hoang Anh, My Tran Ha, Seung Woon Hyeon,
    Hoa Le Thi Quynh, Diep Pham Ngoc, Arslan Sarwar, Seung Gi Kang, Jang Jin Lee, Yu Rim Kim
 Contact :
eunylee@khu.ac.kr , Lab: +82-31-201-3839
 Homepage: https://sites.google.com/view/nanobio-engineering-lab/home

 
     
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